|
KMID : 1145120170150020170
|
|
Asian Journal of Beauty and Cosmetology
2017 Volume.15 No. 2 p.170 ~ p.178
|
|
|
Anti-aging Effects of Diospyros kaki Thunb. Extracts in UVA-irradiated Epidermal Keratinocytes
|
|
Shim Joong-Hyun
Park Sun-Young
|
|
|
Abstract
|
|
|
Purpose: This study aimed to investigate the anti-aging effects of Diospyros kaki Thunb. (DKT) leaf extracts on normal human epidermal keratinocytes (NHEKs).
Methods: To investigate the anti-oxidative effects of DKT leaf extracts on NHEKs, we measured radical-scavenging activity using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. Furthermore, to elucidate the expression of genes and proteins related to skin hydration, we performed a cell viability assay and hyaluronic acid (HA)-enzyme-linked immunosorbent assay (ELISA) and also measured mRNA expression.
Results: We elucidated the effects of DKT leaf extracts on aquaporin 3 (AQP3)/hyaluronan synthase 2 (HAS2)/keratin 1 (KRT1)/keratin 10 (KRT10) mRNA expression and HA production. Real-time polymerase chain reaction (real-time PCR) showed that DKT leaf extracts increased mRNA levels of AQP3/HAS2/KRT1/KRT10, and the HA-ELISA revealed that DKT leaf extracts increased HA production in NHEKs.
Conclusion: The results are indicative of the anti-aging effects of DKT leaf extracts; therefore, DKT leaf extracts may be a potent cosmetic ingredient for anti-aging and skin moisturizing. Based on these findings, we anticipate further research on DKT leaf extracts for mechanisms with a view to developing not only cosmetics but also medicines or healthcare food.
|
|
|
KEYWORD
|
|
|
Diospyros kaki Thunb. leaf, Anti-aging, Hyaluronic acid, AQP3, HAS2
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|